{"id":587,"date":"2014-09-11T01:47:00","date_gmt":"2014-09-11T01:47:00","guid":{"rendered":"http:\/\/suigenerisbrewing.com\/index.php\/2014\/09\/11\/one-mystery-solved\/"},"modified":"2017-09-15T18:25:03","modified_gmt":"2017-09-15T18:25:03","slug":"one-mystery-solved","status":"publish","type":"post","link":"https:\/\/suigenerisbrewing.com\/index.php\/2014\/09\/11\/one-mystery-solved\/","title":{"rendered":"One mystery solved…"},"content":{"rendered":"

As I mentioned yesterday<\/a>\u00a0I recently developed a persistent infection of part of my brewing setup. Thee batches that passed through my primary fermenter ended up infected (based on aroma\/taste\/texture); in the final beer I was able to confirm the infection directly by examining a wort sample in which I observed chains of highly elongated rod-shaped bacteria.<\/p>\n

Aside from having no idea where the infection came from, I also had no idea what it was. The cell size and length:width ratio were too big for lactobacillus<\/i>; the shape and formation of chains was inconsistent with both pediococcus<\/i> and acetobacter<\/i>. In other words, it isn’t likely one of our usual suspects<\/a>. So I ran a variation of the genomic sequencing method I’ve used previously to ID yeast, but this time with primers that recognize bacteria – producing the following sequence:<\/p>\n\n\n\n
AGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGG
\nGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGA
\nGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAA
\nTAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCC
\nGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGC
\nGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGG
\nGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGA
\nTGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGA
\nAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGC
\nTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTG
\nGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGG <\/span>
\nAGCATGTGGTTTAATTCGAAGCA<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

For those who don’t read DNA, that the sequence of\u00a0Bacillus subtilis<\/a><\/i>‘s ribosome. This explains…well pretty much everything. Bacillus<\/i>\u00a0are spore producing species and their spores are very nearly indestructible – they can survive transient exposure to bleach, are pretty much impermeable to sanitizers like starsan, and can even survive short period of boiling! Moreover, their spores stick to surfaces like crazy-glue, making them near-impossible to remove. The persistence of Bacillus<\/i>\u00a0spores, but killing of the vegetative (growing) form after sanitation, would lead to the situation I’ve experienced – stuff fermented briefly in the container appears normal until weeks after packaging, while beers fermented for longer periods show signs while still in the primary.<\/p>\n

Of course, the question is where the heck did this come from? B. subtilis<\/i>\u00a0grows in the soil, and is a normal components of our guts – so it is comforting to think that it may be nothing more than an environmental contaminant. But I don’t think that is the source – at the time I was developing a teaching lab – using\u00a0B. subtilis<\/i>\u00a0– for an undergrad course that I am currently running. \u00a0I have a sneaking suspicion I may literally have brought my work home with me…<\/p>\n","protected":false},"excerpt":{"rendered":"

As I mentioned yesterday\u00a0I recently developed a persistent infection of part of my brewing setup. Thee batches that passed through<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[16,14],"tags":[169,125],"class_list":["post-587","post","type-post","status-publish","format-standard","hentry","category-adventures","category-yeast-wrangling","tag-bacteria","tag-contamination"],"yoast_head":"\nOne mystery solved... - Sui Generis Brewing<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/suigenerisbrewing.com\/index.php\/2014\/09\/11\/one-mystery-solved\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"One mystery solved... - Sui Generis Brewing\" \/>\n<meta property=\"og:description\" content=\"As I mentioned yesterday\u00a0I recently developed a persistent infection of part of my brewing setup. 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