It can be a challenge to form an artefact-free, high contrast image which faithfully reproduces the colour of your sample, with a microscope. The most critical step in forming such an image is properly aligning the condenser of your microscope. <\/p>\n\n\n\n
This video shows how to align an Abbe-style condenser to produce Kholer illumination \u2013 an illumination pattern which limits artefacts and enhances the contrast and colour of your sample. This video demonstrates this procedure and explains the basic physics achieved by Kholer illumination. A much more detailed description of the goal, physics and outcome of Kholer illumination can be found over at Microcourses<\/a>.<\/p>\n\n\n\n