µBrews – Episode 5

It can be a challenge to form an artefact-free, high contrast image which faithfully reproduces the colour of your sample, with a microscope. The most critical step in forming such an image is properly aligning the condenser of your microscope.

This video shows how to align an Abbe-style condenser to produce Kholer illumination – an illumination pattern which limits artefacts and enhances the contrast and colour of your sample. This video demonstrates this procedure and explains the basic physics achieved by Kholer illumination. A much more detailed description of the goal, physics and outcome of Kholer illumination can be found over at Microcourses.

This is part of a video series on using microscopy in the home or craft brewery. A full list of published episodes can be found on my µBrews YouTube playlist, or on my blog.

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