µBrews – Episode 8

Using an adequate pitch rates is critical to ensuring a healthy fermentation, while for some styles of beer, lower pitch rates are needed to ensure the yeast produce the desired flavour profile. Ensuring a correct pitch rate requires the ability to accurately determine the concentration of yeast in a yeast sample. This is done by counting yeast, using a specialized slide called a hemocytometer.

In this video I will explain what a hemocytometer is, how it works, demonstrate its correct usage, and walk you through how to calculate the concentration of yeast in a yeast pitch from hemocytometer counts. At the end of the video I will demonstrate how a hemocytometer can be combined with viability staining (trypan blue staining), thus allowing you to quickly and easily determine both the number of living yeast in your sample and your samples overall viability.

This is part of a video series on using microscopy in the home or craft brewery. A full list of published episodes can be found on my µBrews YouTube playlist, or on my blog.

2 thoughts on “µBrews – Episode 8

  • February 27, 2020 at 5:45 am

    Thanks for teaching.

    I think you misspoke in section 4.41~ (actually 4.48 ~ 4.51) where you say “zero point five” I think you wanted to say “point zero five”.

    the math works better that way i think

    • February 27, 2020 at 1:16 pm

      Yep, that was an error. I’ve added a pinned comment to the video pointing out the correct value.


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